National Programs >> B.Tech. - Biotechnology >> Student Projects




- Detection of mutations (185delAG and R1443X) in the breast cancer susceptibility gene BRCA1 by PCR-mediated site-directed mutagenesis.
- Molecular Characterization of Quinolone Resistance in Salmonella Isolates by PCR-RFLP Technique.
- Lab Scale Production of HS and BQ combined Alum Precipitated vaccine and its safety evaluation.
- Measurement of Uncertainty in Goat Polyclonal Antibody Manufacturing Process in a Biotech Company.
- Defensin Protein Isolation from Raphanus sativus, its characterization and antifungal property.
- Lab Scale Production of PHB using Pseudomonas aeruginosa Isolated from Soil Sample.
- Comparative Phytochemical and Biological Screening of Stevia rebaudiana and Scoparis dulcis.
- Production of Biogas from Waste Cassava Leaf, its Comparative Study with the Kitchen Waste and Re-use of its End Product as a Biofertilizer.


- Multiresidue of pesticide determination in vegetable sample of Kathmandu valley
- Screening, Optimization & Process scale up for Pilot Scale Production of Lipase by Aspergillus niger.
- Multiple shoot production from Allium sativum in MS medium
Phytochemical and antimicrobial property of medicinal plants


- Micropropagation of Mimusops elengi via multiple shooting
Callus culture of Allium sativum
- Cytological study of In vitro cultured potato plant variety
Screening of mutant Streptomyces and their antibiotic production.
- Biodiesel production from microalgae with optimization of growth condition.
- Isolation of essential oil from Artemisia and its antimicrobial assessment.
- Bioprospective analysis of Paris polyphylla found in Himalayan region.
- Isolation of Streptomyces spp. from vermicompost with glucose oxidase enzyme activity


- siRNA delivery via outer membrane vesicle of genetically engineered Escherichia coli for specific and efficient gene knockdown.
- Molecular detection of clinically diagnosed sickle cell cases and it co-existence with alpha-thalassemia in Tharu poupulation.
- Polyclonal antibody production in rabbits using synthetic peptide as antigen.
- Preparation of synthetic seeds in Alium sativum using alginate immobilization technique.
- Production of pectinase by Aspergillus niger isolated from different sites of kathmandu valley and compare the activity of the best strain.
- Screening of microorgnisms for the production of alkaline phosphatase.
- Callus induction and somatic embryogenesis of F1 hybrid tomato seed.
- Estimation of nutrients and anti-nutrients componenets and biological screening of Mango (Mangifera inidca) seed kernel.
- Bioassay of essential oils and crude extracts of ginger (Zingiber officinale ) collected from different regions of Nepal.
- Comparative phytochemical and antimicrobial assessment of ripe berry, unripe berry and flower of Sambucus nigra var. canadensis.
- Toxicity study of crude aerial extract of Euphorbia pulcherrima.


- Isolation of yeast from Murcha and Fruit surface, Comparison of their ethanol production, tolerance and Wine Production. (Deepak Lamsal, Momish Manandhar, Supervised by- Mr. Shishir Sharma, Mrs. Jarina Joshi)
- Production, purification and molecular weight determination ofAlkaline Phosphatase by Aspergillus niger.(Sagar Bhandari, Suman Taming, Supervised by Mr. Shishir Sharma, Mr. Binod Mishra)
- Isolation, screening and biochemical testing of Rhizobium and Azotobacter for the production of Biofertilizer.(Prabesh Singh Chettri, Rishi Bhandari, Supervised by- Mr. Shishir Sharma)
- Isolation and Screening of lytic bacteriophages from environment sources. (Amrit Kafle, Sanjaya Thapa, supervised by Mr. Shishir Sharma)
- Micropropagation of Nepali large cardamom (Amomum subulatum Roxb.) and isolation of its’s Major contaminants during tissue culture. (Yuming Nembang, Sneha Pandey, Supervised by Mr. Hari Krishna Saiju)
- Tissue culture of Stevia rebaudiana and extraction of sweetening compound (glycoside) (Suman Maharjan, Rajita Bhuju, Supervised by- Mr. Hari Krishna Saiju)
- Micropropagation of Asiatic lily. (Ankita Shah, Preetha Kattel, Priyanka Yadav, Supervised by- Mr. Hari Krishna Saiju)
- Isolation of phosphate solubilizing microorganisms. Assessment of their effect on growth and promotion of maize (Zea mays). (Subhiksha Khatiwada, Suruchi Paudel, Supervised by Mrs. Sanu Keshari Bhajracharya)


- Bioleaching effects of bacterial isolates obtained from rust, coal and sewer in low-grade copper ore chalcopyrite. (Adnan Ahmed Shah, Rohit Kaushal, Supervised by- Dr. Ranjan Koirala)
- Characterization of chloropyrifos degrading bacteria isolated from different agricultural field of Nepal.(Roshani Shrestha, Somila Kakshapati, Supervised by Dr. Ranjan Koirala)
- Comparative study of Sheep blood with citrated Human blood and commonly available livestock blood as a substitute in Blood Agar nedium for routine use.(Daisy Awale, Kiran Subedi, Supervised by- Dr. Piyush Rajbhandari)
- Comparative study on the antioxidant and antimicrobial properties of three species of cultivated mushrooms. (Ashmita Timilsina, Srijana Yakkha, supervised by Dr. Mahesh Kumar Adhikari)
- Fungus mediated biosorption of excess copper from soil for increased growth in Glycine max. (Nishan Gautam, Pratik Koirala, Supervised by Mr. Hari Krishna Saiju)
- Isolation and characterization of endophytic fungus for auxin production and study its significance in plant growth. (Rosy Shakya, Shreya Shrestha, Supervised by- Mr. Hari Krishna Saiju, Dr. Ranjan Koirala)
- Isolation and screening of the antibiotic producing microorganism from soil samples of Mustang and Dang. (Chiranjivi Subedi, Laxman Subedi, Supervised by- Mrs. Jarina Joshi)
- Media optimization for multiple shoot induction in large cardamom. (Pushpak Shah, Shashwat Mandal, Supervised by Mr. Hari Krishna Saiju, Mr. Mahesh Maharjan)
- Screening of some nutrients in cultivated mushrooms sold in the market of Kathmandu. (Prakash Gurung, Rekha Bhattarai, Supervised by- Dr. Mahesh Kumar Adhikari)